GCLabs

Search for Test Items

Interferon-gamma releasing assay

GC Labs code	N971	Test Field	Infectious disease

GC Labs code

N971

Test Field

Infectious disease

Information

Test method	EIA (Enzyme immunoassay)
Specimen (mL)	WB 1.0 in each 4 exclusive containers
Specimen storage	Ambient
Specimen stability	Ambient (16 hours)
Test schedule	Mon-Sat
Analytical time (day)	2

Test method

EIA (Enzyme immunoassay)

Specimen (mL)

WB 1.0 in each 4 exclusive containers

Specimen storage

Ambient

Specimen stability

Ambient (16 hours)

Test schedule

Mon-Sat

Analytical time (day)

Reference range/Unit	[Whole Blood] See comments in the report
Clinical Significance	This test is a blood-based interferon-gamma release assay (IGRA) used as an aid in the diagnosis of Mycobacterium tuberculosis infection. It is an immune response-based, indirect test for M tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography, and other medical and diagnostic evaluations. Additional testing is needed to determine if a person who has tested positive has latent tuberculosis (TB) infection or TB disease. This in vitro diagnostic test uses a peptide cocktail simulating ESAT-6, CFP-10, and TB7.7 proteins to stimulate cells in heparinized whole blood. Detection of interferon-γ (IFN-γ) by ELISA is used to identify in vitro responses to those peptide antigens that are associated with Mycobacterium tuberculosis infection.
Compulsory forms
Remark	[Specimen handling method] ① Record patient information and collection time on a dedicated container (4 tubes). ② After collecting blood of 1.0 mL each in the order of gray (Nil) → green (TB1 antigen) → yellow (TB2 antigen) → purple (Mitogen), perform inversion mix 10 times (or repeat for 5 seconds) so that the contents (antigen) on the wall of the dedicated container are mixed well with the blood. ③ After blood collection, the dedicated container must be upright and stored at room temperature, and arrive at the laboratory within 16 hours. [Precautions] ① Comply with specimen volume (collect 1.0 mL of blood in each dedicated container) ② After blood collection, the dedicated container must be upright and stored and transported at room temperature (within 16 hours) ③ Unable to request before a public holiday

Reference range/Unit

 [Whole Blood]
See comments in the report

Clinical Significance

This test is a blood-based interferon-gamma release assay (IGRA) used as an aid in the diagnosis of Mycobacterium tuberculosis infection. It is an immune response-based, indirect test for M tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography, and other medical and diagnostic evaluations. Additional testing is needed to determine if a person who has tested positive has latent tuberculosis (TB) infection or TB disease.

This in vitro diagnostic test uses a peptide cocktail simulating ESAT-6, CFP-10, and TB7.7 proteins to stimulate cells in heparinized whole blood. Detection of interferon-γ (IFN-γ) by ELISA is used to identify in vitro responses to those peptide antigens that are associated with Mycobacterium tuberculosis infection.

Compulsory forms

Remark

[Specimen handling method]
① Record patient information and collection time on a dedicated container (4 tubes).
② After collecting blood of 1.0 mL each in the order of gray (Nil) → green (TB1 antigen) → yellow (TB2 antigen) → purple (Mitogen), perform inversion mix 10 times (or repeat for 5 seconds) so that the contents (antigen) on the wall of the dedicated container are mixed well with the blood.
③ After blood collection, the dedicated container must be upright and stored at room temperature, and arrive at the laboratory within 16 hours.

[Precautions]
① Comply with specimen volume (collect 1.0 mL of blood in each dedicated container)
② After blood collection, the dedicated container must be upright and stored and transported at room temperature (within 16 hours)
③ Unable to request before a public holiday